Table 1. Melting temperatures of antithrombin variants determined by intrinsic fluorescence changes, as described in Materials and Methods section.
Variant | Glycans (n°) | Tm (X ± SD) |
S137A | 3 | 56.2±0.2 |
S137T | 4 | 57.6±0.0 |
K133F/S137A | 3 | 57.3±0.1 |
K133F | 4 | 58.7±0.0 |
K133F/S137T | 4 | 57.3±0.2 |
Each value was calculated as the mean (X) ± standard deviation (SD) of two independent measurements.