Figure 3. Isotype and IgG subclass of HSV-specific antibodies produced by ASCs isolated from lymphoid tissue and non-lymphoid sites of HSV-2 infection.

Hartley guinea pigs were infected ivag with HSV-2 strain MS, serum was collected and lymphocytes were isolated from the indicated tissues on days 48–57 post infection. Supernatants from ASC cultures were collected on day three after ASC isolation. The immunoglobulin isotype and IgG subclass of HSV-specific antibodies from serum and ASC supernatant were determined by ELISA. Each data point represents ASC supernatant from an individual animal. (* P<0.05; ***P<0.0001, Student's t test).