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. 2014 Dec 8;9(12):e114652. doi: 10.1371/journal.pone.0114652

Figure 4. IgG antibody from tissue-resident ASC isolated from HSV-2-infected guinea pigs is reactive with HSV-2 glycoproteins and neutralizes HSV-2.

Figure 4

Hartley guinea pigs were infected ivag with HSV-2 strain MS, serum was collected and lymphocytes were isolated from the indicated tissues between days 48–57 post infection. Supernatants from ASC cultures were collected on day three after isolation and the endpoint titer of antibodies reactive with full-length recombinant HSV-2 gD (rgD2), truncated rgD2, or truncated recombinant HSV-2 rgG (rgG2) was determined by ELISA. (*P<0.05; ** P<0.01; *** P<0.001 compared to HSV-2 gG2 titer). Virus neutralization was detected by incubating a constant virus titer in serial dilutions of ASC supernatant to determine a 50% neutralizing titer (E) or by incubating serial dilutions of HSV-2 virions in undiluted ASC supernatant (F). Results shown are from a representative experiment of three performed.