Table 1. Listing of primers used to examine the influence of the testing material on different cell markers.
Gene | Role | MSC | MSC-OM | Forward primer (5′–3′) | Reverse primer (3′–5′) |
β2MG (NM_004048) | Reference | X | X | TCTCTCTTTCTGGCCTGGAG | CAACTTCAATGTCGGATGGA |
CCND1 (NM_053056.2) | Proliferation | X | CAACAACTTCCTGTCCTACT | GGGTCCATGTTCTGCT | |
Cav-1 (NM_001753.4) | Caveolae-dependent Endocytosis | X | X | ACGTAGACTCGGAGGGACAT | CAGGTCGATCTCCTTGGTGT |
PICALM (NM_001206946.1) | Clathrin-dependent Endocytosis | X | X | ATTTTCTACCCCTAGTTCTTC | AGTAGCAGAGAAAGGATCTC |
Nqo1 (NM_000903.2) | Oxidative stress | X | X | AGCTGGAAGCCGCAGACCTTG | TGAACACTCGCTCAAACCAGCC |
CHOP (NM_001195053.1) | ER stress | X | X | ACCCTGCTTCTCTGGCTTGGC | GGAGAGTGAGGCCTCTGGGAG |
ALP (NM_000478) | Osteogenic Differentiation | X | GGACATGCAGTACGAGCTGA | CCACCAAATGTGAAGACGTG | |
RUNX2 (NM_001015051.3) | Osteogenic Differentiation | X | GGCCTTCAAGGTGGTAGCC | ATCGTTACCCGCCATGAC |
β2MG = beta-2-microglobulin, CCND1 = cyclin D1, Cav-1 = caveolin1, PICALM = phosphatidylinositol binding clathrin assembly protein, Nqo1 = NADPH quinone oxidoreductase 1, CHOP = C/EBP homologous protein, ALP = alkaline phosphatase, RUNX2 = runt-related transcription factor 2.