Skip to main content
. 2014 Dec 8;9(12):e114740. doi: 10.1371/journal.pone.0114740

Table 1. Listing of primers used to examine the influence of the testing material on different cell markers.

Gene Role MSC MSC-OM Forward primer (5′–3′) Reverse primer (3′–5′)
β2MG (NM_004048) Reference X X TCTCTCTTTCTGGCCTGGAG CAACTTCAATGTCGGATGGA
CCND1 (NM_053056.2) Proliferation X CAACAACTTCCTGTCCTACT GGGTCCATGTTCTGCT
Cav-1 (NM_001753.4) Caveolae-dependent Endocytosis X X ACGTAGACTCGGAGGGACAT CAGGTCGATCTCCTTGGTGT
PICALM (NM_001206946.1) Clathrin-dependent Endocytosis X X ATTTTCTACCCCTAGTTCTTC AGTAGCAGAGAAAGGATCTC
Nqo1 (NM_000903.2) Oxidative stress X X AGCTGGAAGCCGCAGACCTTG TGAACACTCGCTCAAACCAGCC
CHOP (NM_001195053.1) ER stress X X ACCCTGCTTCTCTGGCTTGGC GGAGAGTGAGGCCTCTGGGAG
ALP (NM_000478) Osteogenic Differentiation X GGACATGCAGTACGAGCTGA CCACCAAATGTGAAGACGTG
RUNX2 (NM_001015051.3) Osteogenic Differentiation X GGCCTTCAAGGTGGTAGCC ATCGTTACCCGCCATGAC

β2MG  =  beta-2-microglobulin, CCND1  =  cyclin D1, Cav-1  =  caveolin1, PICALM  =  phosphatidylinositol binding clathrin assembly protein, Nqo1  =  NADPH quinone oxidoreductase 1, CHOP  =  C/EBP homologous protein, ALP  =  alkaline phosphatase, RUNX2  =  runt-related transcription factor 2.