Skip to main content
. 2014 Dec 8;9(12):e114725. doi: 10.1371/journal.pone.0114725

Figure 6. Inhibition of GSK3 suppressors cell proliferation in NSCLC cells.

Figure 6

A: NSCLC cells (PC9, H1295 and Hcc193) and type II pneumocytes were cultured for 120 h in the presence or absence of GSK3 inhibitor, CHIR99021 (0, 1, 5 and 10 µM). After 120 h the extent of cell metabolism was assessed using alamar blue. Fluorescence was analysed using a Fusion plate reader and 535 nm excitation and 590 nm emission filters. Each data point represents the average fluorescence over three replicate experiments (mean ± SEM; * indicates p<0.05, ** p<0.001 relative to the growth of type II pneumocytes). B: NSCLC cells (PC9, H1975 and Hcc193) and type II pneumocytes were cultured in the absence and presence of CHIR99021 (0.05, 0.25, 1, 5 and 10 µM). Protein was extracted and lysates were separated on SDS-PAGE gels. Phosphorylation of GS (S641), NFκB p65 (S468) and CRMP (T514) was determined by western blotting. An anti-α-tubulin antibody was used as a control for protein loading.