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. 2014 Dec 8;9(12):e114768. doi: 10.1371/journal.pone.0114768

Figure 3. ZNF300 knockdown abolished megakaryocytic differentiation.

Figure 3

(A) Control and ZNF300 knockdown (shZNF300) cells were cultured in the presence of 10 nM PMA for 72 hours. The morphology of the treated cells was observed under the light microscope (×400 magnifications). (B) The megakaryocytic differentiation of the treated cells was measured by staining cells with PE-conjugated anti-CD61 antibody and analyzed by FACS. (C) The megakaryocyte differentiation of the treated cells was measured by detecting ITGB3 mRNA level (quantitative RT-PCR) and presented as relative expression level. (D) The megakaryocytic differentiation of the treated cells was also measured by detecting ITGA2B mRNA level (quantitative RT-PCR) and presented as relative expression level. Data were representatively results of 3 independent experiments with triplicates. *** indicates p<0.001