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. 2014 Nov 3;5(20):10127–10139. doi: 10.18632/oncotarget.2466

Figure 2. TRIM16 over-expression reduces melanoma cell proliferation and migration.

Figure 2

(A) A panel of melanoma cell lines were transiently transfected with TRIM16 plasmid DNA for 48 hours. Cell proliferation was measured by BrdU incorporation. The lower panel immunoblot confirmed TRIM16 transfection using an anti-Myc tag antibody. A statistically significant difference between empty vector (EV) and TRIM16-transfected cells is indicated. (B) NHEMs were transfected with scrambled siRNA control or two different TRIM16-specific siRNAs for 24 hours. The transwell migration assay was performed using conditioned media as a chemo-attractant. The histogram displays the percentage of the migrated cells divided by the total number of cells initially loaded into the wells. (C) representative phase contrast micrographs of the closure of scratch-wounded confluent cultures of melanoma cells (G-361) transfected with empty vector or TRIM16 plasmid DNA for 48 hours, photographed either immediately or 8 hours after wounding. The histogram displays the proportion of wound closure over 8 hours from the time of wounding.