Figure 7. Effect of GSE on the protein expression of CD44, β-catenin, Oct-4, SNAIL-1 and E-cadherin in CSC enriched CRC colonospheres.
Effect of GSE on the protein expression of, (A) CD44 and β-catenin; (B) OCT-4; and (C) SNAIL-1 and E-cadherin levels in CSC enriched HT29 colonospheres. Stained samples were mounted with DAPI containing anti-fade reagent (Invitrogen) for visualization under confocal microscopy. Z stack analysis was performed and representative scans (X 600 magnification) with individual scan depth of specific colonospheres representing highest fluorescence intensity/signal are shown.