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. 2014 Oct 10;5(20):10151–10169. doi: 10.18632/oncotarget.2467

Figure 8. Effect of GSE on CSC enriched colonosphere formation, induced by no-contact co-culture with adipocytes.

Figure 8

Human type II diabetic visceral pre-adipocytes were grown on cover slips and allowed to differentiate and mature in to human type II diabetic visceral adipocytes (HDP-AC). GSE (10 μg/mL) was used to pre-treat these adipocytes by adding it during differentiation events [chronic treatment: 0-12 days] or after adipocyte maturation [late-acute: day 12 for 48 h]. (A) Matured adipocytes with or without GSE pre-treatment were then used in no-contact HT29 colonosphere assays. After 10 days, the number and size of colonosphere formed was measured. (B) Effect of GSE on the protein expression of CD44 in HDP-AC mediated CSC enriched HT29 colonospheres as observed by immunofluorescence staining. Representative z scan images of immunofluorescence staining (X 400 magnification using confocal microscopy) of colonospheres with CD44-red and DAPI-blue as nuclear stain are shown. Scan depth of specific colonospheres are also shown. (C) Representative photomicrographs (X 200 magnification) of Oil-Red-O (ORO) based identification of differentiated HDP-AC (red lipid droplets), in the absence and presence of GSE exposure are shown. Columns, mean values; error bars, SEM.