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. 2014 Mar 22;5(20):9664–9677. doi: 10.18632/oncotarget.1856

Figure 6. Hypoxia promotes M2 macrophages polarization and tumorigenesis by activating ERK signaling.

Figure 6

RAW264.7 cells were incubated with 20μM PD in the presence or absence of IL6 under hypoxic conditions. (A) Flow cytometric analysis was performed to analyze the percentage of CD209+ cells. (B) Proteins were detected by western-blot analysis. (C) RAW264.7 cells were transfected with siRNAs specifically targeting ERK1/2 or control siRNAs, as described in the Materials and Methods. (C) Flow cytometric analysis of the percentage of CD209+ cells. (D) Proteins were detected by western-blot analysis using specific antibodies. (E) BMDMs were exposed to PD in the presence or absence of IL6. Flow cytometric analysis was used to analyze the percentages of F4/80+CD209+ cells. (F) Colocalization of p-ERK and CD209 was visualized by immunofluorescence staining (G) PD inhibits HUVEC cells tube formation. (H and I) PD inhibits LLC cells migration.