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. 2014 Aug 23;5(20):9727–9743. doi: 10.18632/oncotarget.2359

Figure 2. Reduction of IgG inhibited LPS induced proinflammatory cytokine production in cervical cancer cells.

Figure 2

(A) HeLa cells were transfected with plasmids encoding small RNAs (Control siRNA and IGHG1 siRNA), and selected under 800 μg/ml G418. The efficiency of silencing was evaluated with immunoblot (left panel) or RT-qPCR (right panel). (B) SiHa cells were transfected transiently with IGHG1 shRNA or control shRNA. IgGγ protein levels (left panel) and mRNA levels (right panel) were detected. HeLa cells. (C) and SiHa cells (D) treated with IGHG1 shRNA or control shRNA were stimulated with 100 ng/ml LPS for 20 h. The proinflammatory cytokines (TNF-α, IL-6, and IL-1β) were measured with ELISA (left panel) and RT-qPCR (right panel). Data are presented as means±S. D. of three independent experiments (*P<0.05; **P< 0.01; ***P<0.001).