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. 2014 Oct 31;5(20):9756–9769. doi: 10.18632/oncotarget.2386

Figure 1. Equal effects of NAC and AM on oxidative DNA damage.

Figure 1

IEC-6 cells were incubated with NAC (4 mM) or AM (4 mM) for 60 minutes and irradiated (18 Gy). (A) Measurement of 8-OHdG among the 0 Gy (purple), 18 Gy (red), NAC plus 18 Gy (orange), and AM plus 18 Gy (green) groups was performed using flow cytometry. Cells were fixed 30 minutes after irradiation. (B) Localization of 8-OHdG was assessed by immunofluorescence between different groups before and after irradiation. Cells were fixed 30 minutes after irradiation. (C) Immunohistochemistry of 8-OHdG 24 hours after irradiation in rats pretreatment with vehicle, NAC (200 mg/kg), or AM (200 mg/kg) 30 minutes before 18 Gy whole-abdominal irradiation. Original magnification, 400×. (D) DNA damage was measured using a Comet assay. Cells were analyzed 5 minutes after irradiation.