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. 2014 Oct 31;5(20):9756–9769. doi: 10.18632/oncotarget.2386

Figure 3. p53-dependent radioprotection of IEC-6 cells by AM.

Figure 3

(A) p53 siRNAs were delivered to IEC-6 cells, and mRNA and protein expression were determined at 24 or 48 hours after transfection. Whole cells were used for further experimentation for these conditions. (B) Clonogenic assays were performed in p53-proficient cells. Cells were pretreated with NAC (4 mM) or AM (4 mM) for 60 minutes, and the cells were then irradiated at 0, 6, 12, or 18 Gy for the clonogenic assays. Counting of colonies was performed 6 to 8 days after irradiation. (C) Clonogenic assays were assessed in p53 knockdown cells. IEC-6 cells were used for the clonogenic assays 48 hours after siRNA transfection. Cells were pretreated with AM (4 mM) or vehicle for 60 minutes and then irradiated. The clonogenic assays were performed for three independent experiments for each paired condition (with and without AM). Error bars represent the standard error of the mean. * p < 0.05; ** p < 0.01.