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. 2014 Nov 11;5(20):9770–9782. doi: 10.18632/oncotarget.2390

Table 1. Primer sequences for Methylation-specific PCR (MSP), Pyrosequencing, Real-time PCR and luciferase reporter assay.

Forward Primer Reverse Primer Sequencing Primer
Methylation-specific PCR (MSP)
M-MSP TAG TCG ATT GAA AGT TCG GGC CCT TTC TCG TAA ATC ATT ACG
U-MSP TTT TAG TTG ATT GAA AGT TTG GGT TTT CCC TTT CTC ATA AAT CAT TAC A
Quantitative Bisulfite Pyrosequencing
GGT TTT TTG TAA GGA GAG AGT AGA GAT AAT ATT TTC CCC TTT CCC TTT CT ATA TTT TTG TTA TTT AGT TGT AAG A
Real-time PCR
LT-β GGT TTC AGA AGC TGC CAG AG TTC AGC GGA GCG CCT AT
GAPDH ACC ACA GTC CAT GCC ATC ACT TCC ACC ACC CTG TTG CTG TA
Luciferase Reporter Plasmid
LT-β Wild-type CGA GCT CGG GGA ATA TGA GTG CGT GGT G GCT CTA GAG CTC TAG ATT TAT CGG CAG CAC TGA AGC
LT-β Deletion CCC ATG GCA GTG GGA AAA AGA CTG TTT GGA AAT TG CAA TTT CCA AAC AGT CTT TTT CCC ACT GCC ATG GG