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. 2014 Sep 8;5(20):9823–9837. doi: 10.18632/oncotarget.2450

Figure 1. TM4SF4 expression in lung cancer cell lines is regulated by methylation.

Figure 1

(A) RT-PCR and Western blot analysis of TM4SF4 expression in the indicated lung cancer cell types. Band intensities were measured using Image J software (National Institute of Health, Bethesda, MD, USA), normalized to β-actin and fold increase were indicated. (B) Bisulfite-converted sequence of TM4SF4. Each Y of underlined sequences indicates a methylated position. (C) Pyrosequencing diagram of A549 and H460 cells and comparison of methylation percentages at each CpG position. Each gray box in the diagram indicates the position of the two Y’s shown in panel B. (D) Methylation percentage of TM4SF4 gene in indicated lung cancer cells.