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. 2014 Sep 8;5(20):9823–9837. doi: 10.18632/oncotarget.2450

Figure 3. Overexpression of TM4SF4 in A549 cells enhanced cell growth, migration, and invasiveness.

Figure 3

(A) RT-PCR and Western blot analysis of TM4SF4 expression in pcDNA-TM4SF4-transfected A549 cells: RNA and proteins were extracted from cells 72 h post-transfection. (B) Colony-forming assay of TM4SF4-overexpressing A549 cells: 24 h after transfection, cells were irradiated with a single dose of 2 Gy and, 24 h later, plated for colony-forming assay. (C) Wound-healing assay of TM4SF4-overexpressing A549 cells: A549 cells transfected with pcDNA-TM4SF4 or control vector were used for assay 72 h after transfection. At each period of assay, cells were photographed and wound size was determined. (D) Assay of migration (upper panel) and invasion (lower panel) of TM4SF4-overexpressing A549 cells: A549 cells transfected with pcDNA-TM4SF4 or control vector were used for assay 48 h after transfection.