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. 2014 Sep 16;5(20):9930–9938. doi: 10.18632/oncotarget.2479

Figure 1. Ibrutinib inhibits AML cell migration in response to SDF-1.

Figure 1

(A) AML cell lines were examined for CXCR4 expression (n=4) using flow cytometry (B) AML cell lines were pretreated with 500nM of ibrutinib for 1 h before wash off and then placed in the upper well of a 8.0μM transwell plate. The lower chamber contained 500ul of serum free media supplemented with SDF1 (100 ng/ml) for 3 hours and then assessed for cell number using a flow cytometer. Data were normalised to DMSO treated cells. *statistical significance. (C) Primary AML blasts (n=12) were pretreated with ibrutinib (500 nM) for 1 h before wash off and then placed in the upper well of a 8.0μM transwell plate. The lower chamber contained 500ul of serum free media supplemented with SDF1 (100 ng/ml) for 3 hours and then assessed for cell number using a flow cytometer. Data were normalised to DMSO treated cells.