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. 2014 Sep 16;5(20):9992–10001. doi: 10.18632/oncotarget.2486

Fig.1. Gal-3 expression contributes to cell growth and cell death in thyroid carcinoma cells in response to DXR treatment.

Fig.1

A, Western blot analysis shows Gal-3 protein expression in Nthy-ori 3-1, TPC1 and FTC-133 cells. β-actin was used as the loading control. B, Gal-3 knockdown led to decreased cell growth of FTC-133 cells. Cell growth was analyzed by MTT assay. The value of day 1 was set as 1. **P < 0.01 vs vector. Points in MTT assay represent the mean of three independent experiments; bars, SE. C, FTC-133 and TPC1 stable cells were established as in Material and Methods. They were either untreated or treated with 1 μM DXR for 24 hours. Cell viability was determined by MTT. The value of untreated cells was set as 1. **P < 0.01, *P < 0.05. Columns represent the mean of three independent experiments; bars, SE. D, TPC1 cells were exposed to the indicated concentration of CDDP or DXR. Cell lysates were prepared and processed for western blot assay 24 hours after treatment. E, TPC1 cells were treated with 0.5 μM of DXR. Kinetic analyses of the indicated proteins were done at the times indicated. Analysis was normalized by β-actin. Points represent the mean of two independent experiments; bars, SE.