Table 3.

Multiplexing calculations for the mean and minimum CF and PTG values from Run 1 and Run 2 when the genomic target is the plastome with one copy of the inverted repeat region and a sequencing depth of 30×.

Parameters	Equation abbreviation	Run 1 mean	Run 1 minimum	Run 2 mean	Run 2 minimum
Read length (bp)		100	100	101	101
Total reads generated in a lane		156,000,000	156,000,000	170,464,908	170,464,908
Lane capacity (nucleotides)	LC	15,600,000,000	15,600,000,000	17,046,490,800	17,046,490,800
Reads passing quality filters	CF	0.7960	0.7470	0.8049	0.6733
Proportion mapping to genomic target	PTG	0.0741	0.0305	0.0802	0.0405
Coverage depth desired	CD	30	30	30	30
Target genome size	TaG	124,868	124,868	124,868	124,868
Multiplexing possible	ML	245.5	94.7	293.8	124.2