Table 2. Specific activity of the GSTs from L. migratoria toward six substrates.
Enzyme | CDNB (0.1 mM) | FNDB (1.0 mM) | DCNB (1.0 mM) | NDB-Cl (0.5 mM) | pNPB (0.25 mM) | pNPC (0.5 mM) |
LmGSTD1 | 17.893±0.578 | 125.035±5.295 | 0.098±0.002 | 55.439±1.897 | ND | 0.611±0.033 |
LmGSTD2 | 0.534±0.174 | 110.065±3.135 | 0.286±0.007 | 48.627±0.662 | ND | 0.158±0.008 |
LmGSTD3 | 0.077±0.001 | 1.563±0.067 | 0.023±0.001 | 2.293±0.046 | 0.032±0.000 | 0.051±0.001 |
LmGSTD4 | 5.508±0.099 | 5.019±0.265 | 0.661±0.033 | 3.327±0.151 | 0.072±0.006 | 0.933±0.035 |
LmGSTD5 | 3.003±0.113 | 2.276±0.103 | 0.101±0.004 | 1.897±0.042 | ND | 0.152±0.014 |
LmGSTD6 | 8.530±0.153 | 76.387±1.913 | 0.776±0.016 | 22.999±1.055 | ND | 0.147±0.005 |
LmGSTD7 | 1.024±0.020 | 50.901±1.325 | 0.004±0.001 | 2.379±0.073 | 0.362±0.017 | 0.669±0.060 |
LmGSTE1 | 10.286±0.209 | 0.509±0.049 | 8.811±0.094 | 5.953±0.132 | 0.445±0.040 | 24.318±0.635 |
LmGSTE4 | 43.716±0.758 | 31.436±0.717 | 5.579±0.083 | 16.714±0.260 | 0.068±0.006 | 7.187±0.156 |
LmGSTE5 | 14.806±0.284 | 21.923±0.786 | 8.672±0.290 | 1.830±0.057 | 0.136±0.001 | 1.605±0.019 |
LmGSTS1 | 0.069±0.003 | 1.162±0.035 | 0.006±0.001 | 0.503±0.018 | ND | 0.210±0.003 |
LmGSTS2 | 0.007±0.000 | 1.640±0.069 | ND | 0.153±0.011 | ND | ND |
LmGSTS3 | 2.407±0.050 | 18.730±1.817 | 0.018±0.003 | 1.302±0.001 | ND | ND |
LmGSTS4 | 0.075±0.002 | 8.000±0.144 | 0.005±0.001 | 1.173±0.021 | ND | 0.180±0.050 |
LmGSTS5 | 4.230±0.128 | 15.044±1.018 | 0.022±0.001 | 3.901±0.315 | ND | ND |
LmGSTS7 | 0.360±0.007 | 0.417±0.026 | 0.042±0.001 | 0.061±0.005 | ND | 0.576±0.047 |
LmGSTS8 | 0.090±0.004 | 1.998±0.134 | 0.006±0.001 | 0.343±0.008 | ND | 0.094±0.009 |
LmGSTS9 | 0.829±0.039 | 14.627±0.561 | 0.101±0.003 | 1.096±0.015 | ND | 0.081±0.007 |
LmGSTS10 | 0.167±0.006 | 20.003±1.395 | ND | 0.563±0.019 | ND | 5.113±0.149 |
LmGSTT1 | 0.075±0.003 | 1.474±0.042 | 0.011±0.001 | 0.171±0.004 | 0.334±0.029 | 3.267±0.027 |
LmGSTT2 | 0.548±0.013 | 8.584±0.111 | 0.013±0.002 | 1.446±0.027 | 1.681±0.124 | 19.611±0.469 |
LmGSTO1 | 0.002±0.000 | 0.260±0.014 | ND | 0.339±0.010 | ND | ND |
LmGSTO2 | 0.012±0.001 | 0.493±0.064 | ND | 0.385±0.001 | ND | ND |
LmGSTO3 | 0.005±0.000 | 1.211±0.056 | ND | 0.202±0.011 | ND | ND |
LmGSTZ1 | 0.036±0.001 | 0.327±0.032 | ND | 0.206±0.003 | ND | ND |
The experiment was performed at least five times and the results shown are mean ± SD.
ND, No detectable activity.