Table 3. SteadystatekineticconstantsoftheGSTsfromL.migratoria.
| Enzyme | K m CDNB (mM) | K cat CDNB (s−1) | (K cat/K m)CDNB (mM−1·s−1) | K m GSH (mM) | K cat GSH (S−1) | (K cat/K m)GSH (mM−1·s−1) | |
| LmGSTD1 | 0.139±0.008 | 9,842.92 | 70,740.17 | 0.507±0.025 | 42,664.26 | 84,142.21 | |
| LmGSTD2 | 0.923±0.049 | 89,358.34 | 96,831.84 | 0.226±0.012 | 121,069.26 | 535,015.42 | |
| LmGSTD3 | 0.102±0.010 | 99.08 | 976.10 | 1.977±0.042 | 426.19 | 215.52 | |
| LmGSTD4 | 0.015±0.002 | 3,005.67 | 195,684.54 | 0.356±0.032 | 3,178.80 | 8936.70 | |
| LmGSTD5 | 0.021±0.001 | 13,546.21 | 659,253.89 | 1.595±0.070 | 10,750.18 | 6,741.26 | |
| LmGSTD6 | 0.528±0.301 | 12,675.08 | 24,023.99 | 1.031±0.069 | 35,147.86 | 34,096.99 | |
| LmGSTD7 | 0.492±0.039 | 2,881.65 | 5,856.92 | 0.348±0.012 | 2,648.66 | 7,603.34 | |
| LmGSTE1 | 0.007±0.001 | 13,741.51 | 1,937,102.02 | 1.404±0.099 | 41,709.13 | 29,702.53 | |
| LmGSTE4 | 0.102±0.008 | 33,317.71 | 326,081.31 | 1.239±0.070 | 33,523.92 | 27,058.36 | |
| LmGSTE5 | 0.026±0.002 | 8,356.89 | 324,916.19 | 1.961±0.058 | 9,271.29 | 4,728.43 | |
| LmGSTS1 | 0.226±0.014 | 328.27 | 1,451.66 | 0.748±0.025 | 478.28 | 639.59 | |
| LmGSTS2 | 1.047±0.092 | 1,124.79 | 1074.03 | 0.890±0.042 | 997.15 | 1,120.49 | |
| LmGSTS3 | 0.217±0.023 | 2,030.00 | 9,339.88 | 0.569±0.020 | 3,818.57 | 6,711.74 | |
| LmGSTS4 | 1.292±0.070 | 471.23 | 364.60 | 0.365±0.033 | 392.40 | 1,074.43 | |
| LmGSTS5 | 0.253±0.026 | 6,673.54 | 26,333.24 | 0.555±0.046 | 14,499.79 | 26,138.66 | |
| LmGSTS7 | 0.046±0.001 | 1,730.32 | 37,678.86 | 0.034±0.001 | 8,683.39 | 258,052.90 | |
| LmGSTS8 | 0.100±0.006 | 84.84 | 850.60 | 0.742±0.028 | 251.30 | 338.75 | |
| LmGSTS9 | 0.351±0.026 | 1,646.38 | 4,692.93 | 0.533±0.033 | 3,039.24 | 5,702.23 | |
| LmGSTS10 | 0.311±0.018 | 2,228.91 | 7,172.99 | 1.547±0.076 | 6,555.24 | 4,236.67 | |
| LmGSTT1 | 0.260±0.014 | 996.50 | 3,836.04 | 15.902±1.394 | 26286.68 | 1,653.10 | |
| LmGSTT2 | 0.393±0.049 | 1,203.61 | 3,059.60 | 0.527±0.023 | 1,997.86 | 3,791.14 | |
| LmGSTO1 | 0.228±0.045 | 32.65 | 143.01 | 5.186±0.476 | 114.43 | 27.85 | |
| LmGSTO2 | 0.841±0.138 | 68.10 | 80.98 | 3.301±0.245 | 74.97 | 22.72 | |
| LmGSTO3 | 0.216±0.004 | 221.38 | 1022.63 | 5.577±0.287 | 953.23 | 170.91 | |
| LmGSTZ1 | 0.452±0.058 | 100.01 | 221.47 | 0.531±0.015 | 71.95 | 135.55 | |
The units are as follows: V max, µmol/min/mg; k cat, s−1; K m, mM; k cat/K m, mM−1 s−1.
The data are the means ± SD for at least three independent experiments.