Figure 2. Phase contrast images of ISMCs and MS grown in culture for 3, 7, and 14 days after harvest from neonates.

Smooth muscle cells (top row) were evenly dispersed after seeding and reached confluency at about 14 days in culture. Adherent MS (bottom row) were about 200-µm in diameter with cells emanating outward on the culture surface until reaching confluency after approximately 14 days in culture. 100x magnification, 200-µm scale bar.