Figure 3. Effect of LRRC8A knockdown on kinetics of glutamate (d-[³H]aspartate) release and the intracellular levels of l-glutamate and taurine in astrocyte exposed to hypo-osmotic medium.

A, kinetics of preloaded d-[³H]aspartate release from primary rat astrocytes exposed to 30% reduction in medium osmolarity measured 72 h after treatment with either siNC or siLRRC8A_mix. Data are the means ± s.e.m. of five to six independent experiments performed in two different cell cultures. ***P < 0.001, maximal release rates in siNC vs. siLRRC8A_mix (Student's paired t test). ^#P < 0.05, basal release values in siNC vs. siLRRC8A_mix (two-way ANOVA). B, HPLC analysis of the endogenous l-glutamate and taurine levels in untransfected cells (WT) and cells treated with siNC, or the siLRRC8A_mix. Data are the means ± s.e.m. for three different cell cultures. *P < 0.05; **P < 0.01, hypo-osmotic vs. iso-osmotic conditions for the same treatment (Student's paired t test). hypo, hypo-osmotic; siNC, negative control siRNA; WT, wild-type.