Table 1.

Primers used for engineering DLC1 plasmids

Primer	Sequence (5′–3′)
DLC1-S120A-F	GAGTTTGATGTCTTTGCTCCAAAACAAGAC
DLC1-S120A-R	GTCTTGTTTTGGAGCAAAGACATCAAACTC
DLC1-S120D-F	GAGTTTGATGTCTTTGATCCAAAACAAGAC
DLC1-S120D-R	GTCTTGTTTTGGATCAAAGACATCAAACTC
DLC1-S205A-F	GGCAGCCTGCCCGCTCCCAAGGAACTG
DLC1-S205A-R	CAGTTCCTTGGGAGCGGGCAGGCTGCC
DLC1-S205D-F	GGCAGCCTGCCCGATCCCAAGGAACTG
DLC1-S205D-R	CAGTTCCTTGGGATCGGGCAGGCTGCC
DLC1-S422A-F	GAAAACAGTAGCGACGCCCCCAAGGAACTG
DLC1-S422A-R	CAGTTCCTTGGGGGCGTCGCTACTGTTTTC
DLC1-S422D-F	GAAAACAGTAGCGACGATCCCAAGGAACTG
DLC1-S422D-R	CAGTTCCTTGGGATCGTCGCTACTGTTTTC
DLC1-S509A-F	CCCTGCCCGTCCGCTCCAAAACAGATACAC
DLC1-S509A-R	GTGTATCTGTTTTGGAGCGGACGGGCAGGG
DLC1-S509D-F	CCCTGCCCGTCCGATCCAAAACAGATACAC
DLC1-S509D-R	GTGTATCTGTTTTGGATCGGACGGGCAGGG