. Author manuscript; available in PMC: 2016 Jan 1.

Published in final edited form as: Biochim Biophys Acta. 2014 Oct 12;1848(1 0 0):26–34. doi: 10.1016/j.bbamem.2014.10.007

Table 1.

Summary of quantitative analysis of AFM images

Properties of a ROS disc	Parameter value
	Human	Mouse
Disc diameter	1.17 ± 0.24 μm	1.20 ± 0.25 μm
Inner disc area	0.74 ± 0.37 μm²	0.77 ± 0.39 μm²
Nanodomain height	8.11 ± 0.21 nm	8.10 ± 0.44 nm
Lipid bilayer height	3.68 ± 0.31 nm	3.69 ± 0.28 nm
Number of nanodomains	156 ± 103	156 ± 99
Nanodomain density	208 ± 79 μ⁻²	207 ± 79 μ⁻²
Mean nanodomain size	1,109 ± 311 nm²	1,245 ± 379 nm²
Disc area occupied by nanodomains	22 ± 8 %	24 ± 9 %
Number of rhodopsin molecules	11,642 ± 7,108	13,513 ± 8,522
Rhodopsin spatial density	15,865 ± 5,894 μm⁻²	17,486 ± 6,170 μm⁻²

Parameter values were determined from individual ROS disc membranes. The mean values are reported with the associated standard deviation. The mean was determined from the analysis of 50 human ROS disc membranes or 100 murine ROS disc membranes. Significant differences between human and mouse data was assessed by a t-test. No significant differences between parameters were observed (p > 0.05) except for the mean nanodomain size in a ROS disc membrane, where there is a small but significant difference (p = 0.03).