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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Virology. 2014 Nov 19;474:163–173. doi: 10.1016/j.virol.2014.10.026

Figure 3. Protein levels of apoptosis related genes in PDAC cells following VSV-ΔM51-GFP infection.

Figure 3

Cells were mock-infected or infected with VSV-ΔM51-GFP, VSV-p1-GFP or VSV-GFP at an MOI of 15 (based on BHK-21 titer). At 17 h p.i, cell lysates were prepared and analyzed by Western blot for the indicated proteins associated with (A) virus replication and antiviral response, (B) caspase cleavage, (C) the extrinsic apoptosis pathway, (D) the intrinsic apoptosis pathway and (E) total protein staining. Protein (kDa) product sizes are indicated on the right. Due to a limited amount of total protein isolated from T3M4 and HPAC cells, the following exceptions were made: for PKR T3M4 sample, no protein was loaded for uninfected T3M4 cells; for caspase 3 and FADD in HPAC cells 15 μg (half the amount) was loaded.