Figure 8. Effect of apoptosis inhibition on VSV-ΔM51-GFP replication.
Cells were pretreated with 100 μM caspase-8 inhibitor Z-IETD-FMK, 100 μM general caspase inhibitor Z-VAD-FMK or vehicle only (DMSO diluted in PBS) in growth media for 1 h prior to infection. Cells were then mock infected or infected with VSV-ΔM51-GFP at an MOI of 2 PFU/cell (cell line specific titer) for the study of 1-step replication kinetics based on (A) virus driven GFP expression or (B) infectious particle production (limit of detection=2×102 PFU/ml) as determined at the indicated time points. Assays were performed in triplicate and values represent mean ± standard error of mean. Treatments were compared by two-way ANOVA with Bonferroni posttest for comparison of treatments to control. *, p<0.05; **, p<0.01; ***, p<0.001.