TABLE 2.
Antigen | Antibody | Source | Dilution |
---|---|---|---|
MMP-10 | Goat pAb sc-9941 | Santa Cruz Biotechnology (Dallas, TX) | 1:10 |
Cathepsin F | Goat pAb AF2075 | R&D Systems (Minneapolis, MN) | 1:25 of 0.5 mg/ml stock |
c-Met | Goat pAb sc-161 | Santa Cruz Biotechnology | 1:10 |
Keratin 14 | Mouse mAb NB100-65109 | Novus Biologicals (Littleton, CO) | Undiluted |
Keratin 15 | Mouse mAb sc-47697 | Santa Cruz Biotechnology | 1:10 |
Keratin 17 | Mouse mAb sc-58726 | Santa Cruz Biotechnology | Undiluted |
ANp63 | Goat pAb sc-8609 | Santa Cruz Biotechnology | 1:200 |
Fibronectin | Mouse mAb 618 | Ugarova et al. 1996 | Undiluted |
Laminin γ3 chain | Rabbit pAb R96 | Saghizadeh et al. 2011 | 1:500 |
Nidogen-1 | Mouse mAb MAB2570 | R&D Systems | 1:50 |
Integrin α3β1 | Mouse mAb MAB1992 | EMD Millipore (Billerica, MA) | 1:50 |
p-EGFR (Tyr845) | Rabbit pAb 44-784G | Thermo Fisher Scientific (Waltham, MA) | 1:50 |
p-Akt (Ser473) | Rabbit pAb 9271 | Cell Signaling Technology (Danvers, MA) | 1:50 |
p-p38 (Thr180/Tyr182) | Rabbit pAb 05-1059 | EMD Millipore | Undiluted |
mAb, monoclonal antibody; pAb, polyclonal antibody. Undiluted antibodies refer to straight hybridoma supernatants.