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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Virology. 2014 Nov 14;474:110–116. doi: 10.1016/j.virol.2014.10.013

Figure 1. Subcellular localization of NLS mutant VP2 and VP3 proteins.

Figure 1

(A) VP3 Lysine 200 is conserved in a number of polyomavirus species. (B) Wild type VP2 and VP3 expression plasmids were transfected in RPTE cells, fixed at 24 h, and stained with anti-VP2/3 (red). Nuclei were stained with DAPI (blue). Both VP2 and VP3 are nuclear, as demonstrated by co-staining (bright purple) with DAPI. (C) RPTE cells were transfected with mutant VP3 (VP3K200R) or VP2 (VP2K319R) in the same manner. (D) RPTE cells were co-transfected with VP1 and either VP2 or VP3, and stained for VP2/3, VP1, and DAPI.