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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Oct 23;1848(1 0 0):159–166. doi: 10.1016/j.bbamem.2014.10.022

Figure 2.

Figure 2

Loss of function analysis and Western blots of mutated MscS channels. A) Results from osmotic downshock analysis of wildtype MscS and MscS harboring mutations of lipid lining residues mutated to either alanine or leucine. Error bars represent the standard error of the mean for 6 independent trials. The dark gray bar indicates the construct that is statistically different from wild-type MscS as determined using a Student’s T-test (* = p < 0.05). B) Western blot analysis for wildtype and mutated MscS proteins under conditions identical to those used for the osmotic downshock assay.

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