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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Oct 23;1848(1 0 0):159–166. doi: 10.1016/j.bbamem.2014.10.022

Table 1.

Single channel conductance for MscS mutants and MscL/MscS conductance ratio. Unitary conductance was measured using single channel patch-clamp electrophysiology on spheroplasts at +20 mV under symmetric conditions, error is standard deviation.

Conductance (pS) MscL/MscS
Conductance
Ratio (pS)
Number
of Patches
MscS 1.5 ± 0.3 2.8 ± 0.3 6
V32A 1.7 ± 0.2 3.0 ± 0.6 6
V32L 1.7 ± 0.2 2.7 ± 0.5 6
I43A 1.6 ± 0.5 2.7 ± 0.4 6
I43L 1.2 ± 0.1 3.3 ± 0.2 6
M47A 1.9 ± 0.3 3.1 ± 0.4 6
M47L 1.7 ± 0.2 3.1 ± 0.2 6
N53A 1.6 ± 0.2 3.1 ± 0.6 6
N53L 1.6 ± 0.3 3.2 ± 0.5 6