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. 2014 Nov 3;3(11):e126. doi: 10.1038/oncsis.2014.40

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Copyright © 2014 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Increased numbers of CD8⁺, CD3⁺ and CD4⁺ T cells in tumors generated in Ebag9KO mice. Paraffin sections of tumors generated in Ebag9KO and control mice were blocked in 0.3% _H2O2 and incubated with specific antibodies for CD8 (a), CD3 (b) or CD4 (c). Sections were then incubated with antirabbit EnVision+ reagent and counterstained with hematoxylin. Tumor-infiltrating lymphocytes positive for CD8 (a), CD3 (b) or CD4 (c) expression were microscopically counted in a high-power field of view at a magnification of 400 × (Ebag9KO, n=8; control, n=6). The data shown in lower panels are mean values±s.d. *P<0.05; **P<0.01 by Student's t-test. Scale bar, 20 μm.