Figure 1.

The novel lncRNA ENST00000480739 is downregulated in PDAC. (A) Downregulation of ENST00000480739 was found in 92% (32 of 35) of PDAC tissues. A relative fold change (2^−ΔΔCT) <1/2 was defined as significantly low expression, and >1 was defined as high expression. (B) Expression of mature ENST00000480739 determined by qRT–PCR in 35 paired human PDAC and their corresponding non-tumorous samples (NT) normalised by endogenous β-actin. Paired t-test, P=0.029. (C) Expression level of ENST00000480739 in five pancreatic cancer cell lines and normal pancreatic cell line HPNE. Long non-coding abundance was normalised by endogenous β-actin. Statistical analysis was conducted by one-way analysis of variance, *P<0.05. (D) Relative luciferase assays were performed in SW1990 and HPNE pancreatic cells. Data are shown as mean±s.d., n=3, *P<0.05 (Student's t-test), compared with the control.