Figure 1.

All three AKT isoforms are expressed in BLCA but contribute heterogeneously to active phosphorylated AKT and maintenance of cell viability. (A) Protein expression of the three AKT isoforms was analysed in a panel of cell lines by immunoblotting with the respective antibodies. (B) Representative cell lines were transfected with two different siRNA oligonucleotides against each AKT isoform (A or B) or control (ctrl), and protein expression was analysed by immunoblotting with the respective antibodies. (C) Cell viability of transfected cells was analysed at 72 h. Values indicate mean +/−s.e. expressed as percentage of control from three independent experiments. Statistical comparison was performed using the student t-test (* indicates P<0.05).