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A novel mechanical loading device. (A) A 16-well rubber loading plate of the same dimensions (10 mm diameter) as a standard 48-well tissue culture plate but with a 150 μm thick base and with holes for its attachment to a BOSE loading instrument. (B) Schematic showing the operation of the loading device. The collagen gels are contained in the wells of the silicone plate, and the entire plate is stretched to apply a strain to the gels.
