Table 1.

Primer details.

Gene	Primers (5′–3′)	Amplicon size (bp)
GAPDH (human) NM_002046.5	Fwd – GGT ATC GTG GAA GGA CTC ATG A	68
	Rev – GGC CAT CCA CAG TCT TCT G
HPRT1 (human) NM_000194.2	Fwd – GCA GAC TTT GCT TTC CTT GG	80
	Rev – GTG GGG TCC TTT TCA CCA G
18S rRNA NR_003278.3	Fwd – GCA ATT ATT CCC CAT GAA CG	125
	Rev – GGC CTC ACT AAA CCA TCC AA
E11 (human) NM_006474.4	Fwd – ACG GAG AAA GTG GAT GGA GA	186
	Rev – ACG ATG ATT GCA CCA ATG AA
RUNX2 (human) NM_ 001024630.3	Fwd – GTG GAC GAG GCA AGA GTT TC	107
	Rev – TTC CCG AGG TCC ATC TAC TG
COL1A1 (human) NM_000088.3	Fwd – CAG CCG CTT CAC CTA CAG C	83
	Rev – TTT TGT ATT CAA TCA CTG TCT TGC C
OCN (human) NM_199173.4	Fwd – GGC AGC GAG GTA GTG AAG AG	73
	Rev – GAT CCG GGT AGG GGA CTG
Gapdh (mouse) NM_ 001289726.1	Fwd – GAC GGC CGC ATC TTC TTG TGC A	114
	Rev – TGC AAA TGG CAG CCC TGG TGA C
Hprt (mouse) NM_013556.2	Fwd – CGTGATTAGCGATGATGAACCAGGT	149
	Rev – CCATCTCCTTCATGACATCTCGAGC
E11 (mouse) NM_010329.3	Fwd – AAG ATG GCT TGC CAG TAG TCA	118
	Rev – GGC GAG AAC CTT CCA GAA AT
Runx2 (mouse) NM_ 001146038.2	Fwd – GAC GAG GCA AGA GTT TCA CC	120
	Rev – GTC TGT GCC TTC TTG GTT CC
Col1a1 (mouse) NM_007742.3	Fwd – ACT GCC CTC CTG ACG CAT GG	140
	Rev – TCG CAC ACA GCC GTG CCA TT
OCN (mouse) NM_007541.3	Fwd – CCGCCTACAAACGCATCTAT	153
	Rev – TTTTGGAGCTGCTGTGACAT
ALP (mouse) NM_ 001287172.1	Fwd – GCTGGCCCTTGACCCCTCCA	132
	Rev – ATCCGGAGGGCCACCTCCAC
SV40 YP_003708382.1	Fwd – AGGGGGAGGTGTGGGAGGTTTT	100
	Rev – TCAGGCCCCTCAGTCCTCAC