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. 2014 May 23;32(6):1380–1389. doi: 10.1002/stem.1661

Table 2.

CD34 antibody selection: epitope specificity and clones

Epitope class Clone Cell type Assays performed Reference
Ia. Sensitive to neuraminidase and glycoprotease 12.8 Hematopoietic cells, MSC, endothelial progenitors Immunoblots, immunostaining, FACS 19, 47
BI.3C5 MSC, keratocytes, endothelial progenitors Immunoblots, immunostaining, FACS 1, 9, 75
Ib. Partially sensitive to neuraminidase and glycoprotease ICH3 MSC, keratocytes, endothelial progenitors Immunoblots, immunostaining, FACS 1, 9, 75, 102
MY10 Hematopoietic cells, MSC, interstitial cells, endothelial progenitors Immunoblots, immunostaining, FACS, Western blots 1, 5, 9, 36, 39, 56, 84
II. Resistant to neuraminidase and sensitive to glycoprotease QBEnd10 MSC, muscle satellite cells, keratocytes, interstitial cells, HF epithelial progenitors, endothelial progenitors. Immunoblots, immunostaining, flow cytometry 1, 16, 23, 25, 65, 77, 86, 89, 101, 102
III. Resistant to neuraminidase and glycoprotease 563 HF epithelial progenitors Immunostaining 90
581 Muscle satellite cells, keratocytes Immunostaining, flow cytometry 16, 75
8G12 Muscle satellite cells, haematopoietic cells, MSC Immunostaining, flow cytometry, FACS 8, 12, 40, 44, 62, 64, 73
TUK3 Endothelial progenitors Immunoblots 1
115.2 Endothelial progenitors Immunoblots 1
Monoclonal (clone not stated) MSC Immunostaining, flow cytometry 10, 58, 59, 63, 69
Polyclonal HF epithelial Flow cytometry 91
Antibody type not stated MSC, muscle satellite cells, keratocytes, HF epithelial progenitors, salivary epithelial progenitors, endothelial progenitors Immunostaining, flow cytometry, FACS 1315, 18, 21, 30, 34, 60, 67, 68, 70, 74, 82, 83, 92, 94

Abbreviations: FACS, fluorescence-activated cell sorting; HF, hair follicle; MSC, multipotent mesenchymal stromal cells.