Table 2.
CD34 antibody selection: epitope specificity and clones
| Epitope class | Clone | Cell type | Assays performed | Reference |
|---|---|---|---|---|
| Ia. Sensitive to neuraminidase and glycoprotease | 12.8 | Hematopoietic cells, MSC, endothelial progenitors | Immunoblots, immunostaining, FACS | 19, 47 |
| BI.3C5 | MSC, keratocytes, endothelial progenitors | Immunoblots, immunostaining, FACS | 1, 9, 75 | |
| Ib. Partially sensitive to neuraminidase and glycoprotease | ICH3 | MSC, keratocytes, endothelial progenitors | Immunoblots, immunostaining, FACS | 1, 9, 75, 102 |
| MY10 | Hematopoietic cells, MSC, interstitial cells, endothelial progenitors | Immunoblots, immunostaining, FACS, Western blots | 1, 5, 9, 36, 39, 56, 84 | |
| II. Resistant to neuraminidase and sensitive to glycoprotease | QBEnd10 | MSC, muscle satellite cells, keratocytes, interstitial cells, HF epithelial progenitors, endothelial progenitors. | Immunoblots, immunostaining, flow cytometry | 1, 16, 23, 25, 65, 77, 86, 89, 101, 102 |
| III. Resistant to neuraminidase and glycoprotease | 563 | HF epithelial progenitors | Immunostaining | 90 |
| 581 | Muscle satellite cells, keratocytes | Immunostaining, flow cytometry | 16, 75 | |
| 8G12 | Muscle satellite cells, haematopoietic cells, MSC | Immunostaining, flow cytometry, FACS | 8, 12, 40, 44, 62, 64, 73 | |
| TUK3 | Endothelial progenitors | Immunoblots | 1 | |
| 115.2 | Endothelial progenitors | Immunoblots | 1 | |
| Monoclonal (clone not stated) | MSC | Immunostaining, flow cytometry | 10, 58, 59, 63, 69 | |
| Polyclonal | HF epithelial | Flow cytometry | 91 | |
| Antibody type not stated | MSC, muscle satellite cells, keratocytes, HF epithelial progenitors, salivary epithelial progenitors, endothelial progenitors | Immunostaining, flow cytometry, FACS | 13–15, 18, 21, 30, 34, 60, 67, 68, 70, 74, 82, 83, 92, 94 | |
Abbreviations: FACS, fluorescence-activated cell sorting; HF, hair follicle; MSC, multipotent mesenchymal stromal cells.