Fig. 2.

5-HD attenuates DDFPe induced cardiac protection. Mice were pretreated with 5-HD prior to LAD ligation and saline vehicle or DDFPe administration. Hearts were perfused with trypan blue 24 h after LAD ligation and harvested for transverse sectioning and TTC staining (a) for assessment of the area at risk (b) and infarct size (c). Prior to trypan blue perfusion, heparin treated blood was collected for plasma preparation and cTnI ELISA analysis (d). n = 6–8. Means ± SEM are indicated next to each group in grey. N.S. indicates that means were compared using Student’s t-test Mann–Whitney rank sum were not significantly different