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. 2014 Jun 18;234(1):11–22. doi: 10.1002/path.4349

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© 2014 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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CD133-expressing GSCs locate closely to Shh-expressed endothelial cells in human GBM samples. (A) CD133⁺ tumour cells (green) located close to CD31⁺ endothelial cells (red). (B) CD31⁺ endothelial cells expressed Shh (orange, arrow). (C) CD133⁺ tumour cells (red, arrow) located next to Shh-expressed cells (green). (D) Gli1-expressed tumour cells (green, arrow) closely located to CD31⁺ endothelial cells (red). (E) CD31 and VE-cadherin were co-expressed in endothelial cells (yellow). (F) CD31⁺ endothelial cells (green) were not CD45⁺ (red, arrow). Right panels are partial enlargements of the corresponding left panels.