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. 2014 Jun 18;234(1):11–22. doi: 10.1002/path.4349

Figure 3.

Figure 3

Endothelial cells up-regulate expression of CSC-associated genes in glioma cells. As compared to GL261 alone, expression of GSC-associated genes Olig2, Bmi1 and Sox2 were elevated in GL261 cells co-cultured with b.END3 cells: (A) real-time RT–PCR, *p < 0.05; (B) western blot, tubulin was used as control. As compared to U251 cells alone, expressions of Olig2, Bmi1 and Sox2 were elevated in U251 cells co-cultured with HUVECs; (C) real-time RT–PCR, *p < 0.05; (D) western blot, tubulin was used as control. (E) Immunofluorescence staining revealed that expressions of Bmi1 (upper), Sox2 (middle) and Olig2 (lower) were increased in GL261 cells co-cultured with b.END3 cells. Right panels are partial enlargements of the corresponding left panels.