Table 4.
Summary of putative adaptive candidates detected across multiple methods in at least one of three subsets of data: lodgepole pine (Pinus contorta var. latifolia), jack pine (Pinus banksiana), and combined, including diversity measures (observed heterozygosity (HO), expected heterozygosity (UHE), and fixation index (F)), their estimated linkage group, the number of positive outlier tests for each data set, the direction of selection, the putative amino acid (AA) change (I, intragenic; NC, noncoding; NS, nonsynonymous; S, synonymous), and their putative annotation
| Locus | Lodgepole pine | Jack pine | Linkage group | Outlier detection | Introgress P-value | Direction | AA change | Annotation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N | Ho | UHe | F | N | Ho | UHe | F | Combined | Lodgepole | Jack | ||||||
| JpLpc45225p571 | 368 | 0.043 | 0.043 | −0.022 | 99 | 0.202 | 0.183 | −0.112 | LP07 | 1 | 3 | 0.002 | Positive | NS | B-cell receptor-associated protein 31-like | |
| Lodgc1087p211 | 368 | 0.239 | 0.270 | 0.112 | 100 | 0.000 | 0.000 | LP13 | 1 | 3 | 0.005 | Positive | NS | Proteasome alpha type 7 | ||
| Lodgc2304p514 | 367 | 0.240 | 0.274 | 0.123 | 100 | 0.010 | 0.010 | −0.005 | LP14 | 1 | 3 | 0.240 | Positive | NS | Triosephosphate isomerase | |
| Jp_c01249p362 | 349 | 0.610 | 0.425 | −0.439 | 93 | 0.516 | 0.466 | −0.113 | JP03 | 2 | 0.000 | Negative | NC | Short-chain dehydrogenase-reductase B/KR domain | ||
| Jp_c21224p439 | 368 | 0.402 | 0.381 | −0.057 | 100 | 0.350 | 0.351 | −0.004 | 2 | 0.547 | Negative | S | Transketolase/dehydrogenase-5-phosphate synthase | |||
| Jp_c25075p377 | 368 | 0.416 | 0.419 | 0.005 | 100 | 0.560 | 0.451 | −0.248 | 2 | 0.000 | Negative | S | Phenylcoumaran benzylic ether reductase/ NmrA-negative transcriptional regulator | |||
| JpLpc33893p239 | 368 | 0.016 | 0.016 | −0.008 | 100 | 0.070 | 0.104 | 0.327 | 3 | 0.585 | Positive | NC | Auxin-F-box protein 5 | |||
| JpLpc41319p340 | 368 | 0.027 | 0.032 | 0.153 | 100 | 0.030 | 0.049 | 0.385 | 2 | 1 | 0.992 | Positive | NC | Uncharacterized BCR, YbaB family COG0718 | ||
| JpLpc47089p1831 | 366 | 0.016 | 0.016 | −0.008 | 100 | 0.190 | 0.173 | −0.105 | 2 | 1 | 0.249 | Positive | NC | Dof-type zinc finger DNA-binding family protein | ||
| JpLpc63491p915 | 368 | 0.019 | 0.019 | −0.010 | 100 | 0.240 | 0.256 | 0.059 | 2 | 1 | 0.002 | Positive | NC | Cytokinin response factor/AP2 domain | ||
| JpLpc66545p1207 | 368 | 0.019 | 0.019 | −0.010 | 99 | 0.081 | 0.078 | −0.042 | 2 | 1 | 0.020 | Positive | S | Transcribed locus | ||
| Lp_c00150p459 | 367 | 0.014 | 0.014 | −0.007 | 100 | 0.130 | 0.173 | 0.244 | 2 | 0.353 | Positive | I | Circadian clock associated 1 | |||
| Lodgc118p350 | 365 | 0.427 | 0.467 | 0.083 | 100 | 0.010 | 0.010 | −0.005 | LP13 | 2 | 3 | 0.000 | Positive | S | Sedoheptulose-bisphosphate/fructose-1-6-bisphosphate – chloroplast like | |
| Jp_c13093p749 | 364 | 0.025 | 0.024 | −0.013 | 100 | 0.550 | 0.496 | −0.113 | LP04/JP11 | 2 | 0.001 | Positive | NC | HSP20-like chaperones superfamily protein | ||
| Jp_c13875p1478 | 367 | 0.071 | 0.068 | −0.037 | LP05 | 2 | na | Positive | S | Pectin methylesterase | ||||||
| Jp_c23409p668 | 368 | 0.024 | 0.024 | −0.012 | 100 | 0.560 | 0.499 | −0.127 | 2 | 0.205 | Positive | S | Glyoxalase/bleomycin resistance protein/dioxygenase superfamily | |||
| Jp_c24821p611 | 368 | 0.027 | 0.027 | −0.014 | 100 | 0.320 | 0.284 | −0.134 | 1 | 2 | 0.494 | Positive | S | Nodulin MtN3 family protein | ||
| Jp_c43213p458 | 368 | 0.019 | 0.019 | −0.010 | 100 | 0.900 | 0.499 | −0.812 | LP07 | 2 | 0.000 | Positive | S | Lipid transfer protein/seed storage 25 albumin superfamily protein | ||
| Jp_c44637p536 | 368 | 0.022 | 0.022 | −0.011 | 100 | 0.470 | 0.502 | 0.059 | LP07 | 2 | 0.579 | Positive | NC | Coatomer alpha subunit/glycine-rich protein | ||
| Jp_c44933p494 | 368 | 0.024 | 0.024 | −0.012 | 100 | 0.390 | 0.410 | 0.043 | 1 | 2 | 0.139 | Positive | S | Photosystem II reaction center protein | ||
| JpLpc15608p1838 | 337 | 0.151 | 0.140 | −0.082 | 3 | na | Positive | NC | Transducin family protein/WD-40 repeat family protein | |||||||
| JpLpc21364p981 | 368 | 0.052 | 0.056 | 0.069 | 100 | 0.140 | 0.131 | −0.075 | 1 | 3 | 0.000 | Positive | S | myb-like DNA binding domain | ||
| JpLpc44782p470 | 368 | 0.022 | 0.022 | −0.011 | 100 | 0.320 | 0.297 | −0.084 | 1 | 2 | 0.335 | Positive | S | KNOX1/2 domain/KNOTTED-like | ||
| Lp_c04318p2154 | 368 | 0.207 | 0.215 | 0.038 | 100 | 0.000 | 0.000 | LP14 | 3 | 0.267 | Positive | NC | Carbohydrate binding molecule/starch branching enzyme 2.2 | |||
| Jackc1504p209 | 368 | 0.003 | 0.003 | −0.001 | 100 | 0.300 | 0.309 | 0.025 | 2 | 0.002 | Positive | S | Transcribed locus | |||
| Jp_c28904p670 | 368 | 0.011 | 0.011 | −0.005 | 100 | 0.350 | 0.488 | 0.279 | 2 | 0.000 | Positive | NC | Ubiquitin-conjugating enzyme 28//E3 interaction residues | |||
| Jp_c34152p781 | 347 | 0.127 | 0.124 | −0.024 | 99 | 0.515 | 0.502 | −0.031 | 2 | 0.018 | Positive | S | Ribosomal protein L27 | |||
| Lodgc6568p554 | 368 | 0.329 | 0.366 | 0.100 | 100 | 0.100 | 0.131 | 0.232 | JP09 | 2 | 0.010 | Positive | S | Flavanone 3-hydroxylase | ||
Bold values indicate P-values that are significant following correction for FDR. na, not available.