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. 2014 Nov 26;14:114. doi: 10.1186/s12935-014-0114-8

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© Wu et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The 5′-flanking sequence of VH6-1 exhibits promoter activity in non-B cancer cells. (A) Schematic diagram of 5′-flanking 1.2-kb pGL3 construct. (B) The 1.2-kb fragments amplified from upstream of VH6-1 in HT-29 cells by PCR. (C) The 1.2-kb pGL3 construct was transfected into HeLa, HeLa MR, HeLa S3, HT-29, MDA-MB-231, HEK 293,L02, HK2, Raji or Jurkat cells. Luciferase activity was measured using a dual-luciferase reporter system. The results are representative of three independent experiments after normalization to renilla luciferase activity (internal controls). Each bar represents mean ± SD.