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Cysteamine disrupts production of normal human bronchial epithelial (NHBE) cell-derived mucus. Differentiated NHBE monolayers were exposed (basally) to 1 mg/ml cysteamine or control culture media for 7 days. Mucin production at the apical aspect was then assessed by Alcian blue staining macro- and microscopically (panels A & B) and the amount of free, non-mucin bound airway surface fluid quantified (panel C).
