Table 2.
Linearity, intra- and interday accuracy and precision of analytical method for NAE measurement from brain matrix.
| Analyte | R2 | Nominal concentration (ng/mL) | Intraday 1 (n=3) |
Intraday 2 (n=3) |
Interday (n=8) |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean (ng/mL) | Accuracy (%) | CV (%) | Mean (ng/mL) | Accuracy (%) | CV (%) | Mean (ng/mL) | Accuracy (%) | CV (%) | |||
| AEA | 0.999 | 7.5 | 8.6 | 115.0 | 9.4 | 7.9 | 105.8 | 1.3 | 8.2 | 109.5 | 7.8 |
| 35 | 31.7 | 105.7 | 6.2 | 34.2 | 114.0 | 6.1 | 33.2 | 110.8 | 7.5 | ||
| 75 | 68.1 | 90.8 | 7.5 | 72.5 | 96.7 | 9.1 | 68.5 | 91.4 | 8.2 | ||
| OEA | 0.989 | 15 | 18.2 | 121.5 | 18.8 | 14.8 | 98.4 | 5.5 | 15.9 | 105.8 | 17.6 |
| 30 | 30.9 | 102.9 | 5.1 | 25.1 | 83.6 | 2.8 | 28.4 | 94.8 | 13.3 | ||
| 90 | 85.8 | 95.4 | 5.3 | 90.6 | 100.7 | 4.2 | 85.9 | 95.4 | 6.1 | ||
| PEA | 0.999 | 15 | 14.9 | 99.4 | 11.6 | 13.9 | 93.0 | 3.6 | 14.3 | 95.0 | 8.6 |
| 30 | 28.5 | 95.0 | 13.2 | 29.1 | 97.1 | 7.2 | 28.6 | 95.8 | 8.7 | ||
| 90 | 94.8 | 105.3 | 5.4 | 80.7 | 89.6 | 4.9 | 88.9 | 98.8 | 8.9 | ||