Figure 1.

Structure of YTH-YTHDF2 in complex with an m⁶A mononucleotide. (A) Structural superimposition of free (colored in palecyan) and m⁶A-bound YTH-YTHDF2 (colored in blue) structures. The m⁶A mononucleotide is shown in yellow stick. (B) Electrostatic potential surface of YTH-YTHDF2 in complex with the m⁶A mononucleotide. (C) Detailed interactions between YTH-YTHDF2 and the m⁶A mononucleotide. The weighted 2F_o-F_c electron density map of m⁶A is shown. (D) FP assays of wild type and mutants of YTH-YTHDF2 incubated with m⁶A-containing RNA probe or unmethylated RNA probe. (E) Sequence alignment of the YTH domains of Homo sapiens YTHDF1-3, YTHDC1 and YTHDC2, and S. cerevisiae PHO92. Residues forming the aromatic cage are indicated with red triangles and residues involved in hydrogen bond interactions with m⁶A are indicated with cyan triangles. The structure figures were prepared with PyMOL.