Table 2.
Percentage of cells with n foci
|
Avg. no. of foci | |||||||
---|---|---|---|---|---|---|---|---|
Strain (% xylose) | 2-AP | No. of cells | 0 | 1 | 2 | 3 | >4 | per cell |
DnaE-GFP (0.125%) | − | 1082 | 15.9 | 52.9 | 23.2 | 7.0 | 1.0 | 1.24 |
+ | 1003 | 47.0 | 41.3 | 9.7 | 1.6 | 0.3 | 0.67 | |
DnaE-GFP (0.025%) | − | 962 | 36.4 | 43.7 | 17.2 | 2.6 | 0.2 | 0.87 |
+ | 1414 | 56.3 | 37.0 | 6.4 | 0.4 | 0 | 0.51 | |
DnaE-GFP (0.005%) | − | 1040 | 54.6 | 34.4 | 9.3 | 1.5 | 0 | 0.58 |
+ | 1671 | 80.3 | 18.4 | 1.3 | 0 | 0 | 0.21 |
Strains were grown in defined S750 minimal medium with 1% arabinose with the amount of xylose indicated in parentheses. Cultures were split, followed by the addition of 2-AP (600 μg/mL) to one of the cultures for 1 hr prior to imaging (“Experimental Procedures”).