Figure 3.

Effects of the BKCa channel opener NS1619 on K⁺-current recorded in SH-SY5Y neuronal cell line. The effects of the NS1619 (10 × 10⁻⁶ M and 100 × 10⁻⁶ M) were investigated on the K⁺-current recorded in asymmetrical K⁺ ions concentrations (int K⁺: 132 × 10⁻³ M; ext K⁺: 2.8 × 10⁻³ M), in the presence of internal 1.6 × 10⁻⁶ M concentration of free Ca²⁺ ions, in the range of potentials going from −150 to +110 mV, HP = −60 mV using whole cell patch clamp technique. The K⁺-current was leak subtracted and normalized to capacitance. The I/V relationship was constructed in the absence (CTRL) or presence of different concentrations of NS1619. In the this experiment, the application of 10 × 10⁻⁶ M and 100 × 10⁻⁶ M concentrations of this drug enhanced the K⁺-channel current, at −10 mV (Vm), respectively by +41.7 and +171% with respect to the control.