Figure 6.

PKC and PKA activities in SH-SY5Y neuronal cell after 6 h incubation in the presence of the channel blockers IbTX and TEA and opener NS1619. The effects of the BKCa channel modulators on the protein kinase C (PKC) and protein kinase A (PKA) were investigated in the cell lysate. The cells were incubated with the drugs under investigation for 6 h and the cell lysate were analyzed using an ELISA assay for PKC and PKA activities (A) IbTX (10 × 10⁻⁹ M), TEA (100 × 10⁻⁶ M), and NS1619 (50 × 10⁻⁶ M) significantly enhanced PKC activity, while the STS (0.2 × 10⁻⁶ M) reduced it with respect to the controls (B) TEA, IbTX, and NS1819 enhanced PKA activity, while STS was without effect with respect to the controls. ^*Data significantly different with respect to the controls for p < 0.05 as determined by student t-test.