Fig. 6.

ROR1 antibody UC-961 can inhibit spheroid formation of ROR1⁺ cancer cells. (A, Left) Representative images of spheroids formed by AA0857 ovarian cancer cells cultured for 3 wk in media containing control hIgG or UC-961, each at 50 µg/mL. (Scale bar: 100 µm.) Average numbers of spheroids formed in each of three separate wells with either treatment (±SEM) are indicated in the bar graph (Right), as in Fig. 3D. (B) Graph showing the average numbers of detected spheroids over time in three separate wells with either treatment (±SEM). (C) Confocal microscopy of ovarian cancer cell spheroids stained with mAb specific for ROR1 (green) or vimentin (red), after treatment with UC-961 for 48 h. Nuclear staining is in blue. (Scale bar: 10 μm.)